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Molisch Test:
Shows positive test for:
All carbohydrates, Monosaccharides,disaccarides,and polysacarides,shouhd give a positive reaction.nucleic acids and glycoproteines also give a positive reaction.
Molishs reagent:
2g 1-naphthol dissolved in 20 ml etoh 95%
Reactions:
all these compounds are eventually hydrolysed to monosacharides by strongmineral acids.
The test reagent dehydrates pentoses to form furfural and dehydrates hexoses to form 5-hydroxymethyl furfural .The furfurals further react with -naphthol present in the test reagent to produce a purple product.
Procedure:
Two ml of a sample solution is placed in a test tube. Two drops of the Molisch reagent (a solution of -napthol in 95% ethanol) is added. The solution is then poured slowly into a tube containing two ml of concentrated sulfuric acid so that two layers form.
A positive test is indicated by:
The formation of a purple product at the interface of the two layers.
doing this tommorrow in lab, hopefully this will help.
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